Parti 2: Proprjetajiet Anti-infjammatorji U Antiossidanti Tal-Millieġ tas-Saba' (Eleusine Coracana (L.) Gaertn.) Varjetajiet Ikkultivati ​​fis-Sri Lanka

Għal aktar informazzjoni. kuntatttina.xiang@wecistanche.com

Infjammazzjonihuwa rispons immuni tal-ġisem tagħna biex jiġġieled l-infezzjoni u jsewwi t-tessut bil-ħsara; huwa proċess ta 'awto-fejqan u fejqan ikkawżat minn ċerta marda fil-ġisem tagħna.

Ħafna mard tal-ġisem tal-bniedem huwa relatat ma 'infjammazzjoni. Pereżempju, studji wrew li l-kawża ewlenija tal-mard tal-qalb hija infjammazzjoni arterjali, bħal osteoartrite varji, infjammazzjoni ġinekoloġika, gastroenterite, epatite kronika, koleċistite, nefrite, pnewmonja, u anke rinite. , keratite, otitis media...anke kanċer, jista 'jingħad li kważi l-mard kroniku kollu huwa relatat ma' infjammazzjoni.

Meta batterja jew virus ta 'ħsara jattakkana, is-sistema ta' validazzjoni tal-ġisem tagħna tibda u tiġġieled lura; jew jekk aħna sprain għaksa tagħna, is-sistema infjammatorja tagħna kicks biex isewwi t-tessut bil-ħsara. Mingħajr is-sistema infjammatorja, imutu malajr.

Studji sperimentali wrew li echinacea b'ħafnaCistanche deserticolaestratt għandu effett tajjeb ta 'titjib fuq ġrieden kolite. Il-komposti tal-phenethylgly iżolati minn Cistanche għandhom effett protettiv fuq iċ-ċelloli infjammatorji kkawżati mill-monossidu tal-karbonju. Il-glukosidi totali ta 'Cistanche għandhom effett inibitorju tajjeb fuq is-sekrezzjoni tal-monossidu tal-karbonju fiċ-ċelloli infjammatorji, u r-riżultati juru li għandu effett anti-infjammatorju. Barra minn hekk, Cistanche kien toniku tajjeb minn żminijiet antiki, u m'hemm l-ebda rekord ta 'tossiċità, li juri bis-sħiħ is-sigurtà tiegħu.

3. Riżultati u Diskussjoni

3.1.Proprjetajiet Antiossidanti tal-Varjetajiet tal-Millieġ tas-Saba '

3.1.1.Kontenut totali ta 'Fenoliċi u Flavonojdi Totali.TPC u TFC ta 'estratti etanoliċi u metanoliċi ta' varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha huma ppreżentati fit-Tabella 1. Kien hemm differenzi sinifikanti (P<0.05)among the="" varieties="" and="" between="" the="" extracts.="" methanolic="" extracts="" had="" significantly=""><0.05)tpcs and="" tfcs="" compared="" to="" ethanolic="" extracts="" in="" all="" finger="" millet="" varieties.="" among="" the="" three="" varieties,="" oshadha="" had="" a="" significantly="" high=""><0.05)tpc, and="" ravi="" had="" a="" significantly=""><0.05 )tfc.="" there="" were="" no="" significant="" differences(p≥0.05)between="" the="" tfcs="" of="" rawana="" and="">

Negattiv sinifikanti (P<0.05) correlations="" were="" observed="" between="" tpcs="" and="" ics.="" values="" of="" dpph="" (r="-0.602)" and="" abts="" cation(r="-0.836)radical" scavenging="" activities="" of="" all="" extracts.in="" addition,a="" significant="" positive=""><0.05) correlation="" was="" observed="" between="" tpcs="" and="" orac="" values(r="0.787)" of="" the="" extracts.="" these="" correlations="" indicated="" the="" involvement="" of="" finger="" millet="" phenols="" in="" radical="" scavenging="" activities.="" tpcs="" of="" the="" extracts="" positively="" correlated="" with="" frap="" values(r="0.876,"><0.05)and fic="" activities(r="0.473,"><0.05)of the="" extracts="" indicating="" the="" ability="" of="" finger="" millet="" phenols="" to="" reduce="" ferric="" ions="" and="" chelate="" ferrous="" ions.="" there="" were="" significant=""><0.05)correlations between="" tfcs="" and="" ic5="" values="" of="" dpph(r="-0.802)and" abts="" cation(r="-0.471)radical" scavenging="" activities="" of="" the="" extracts.="" besides,="" tfcs="" positively="" correlated="" with="" fic="" activities(r="0.689,"><0.05)of the="" extracts,="" suggesting="" the="" involvement="" of="" finger="" millet="">flavonojdi fit-tneħħija tar-radikali u l-joni tal-ħadid kelanti.

TPCs ta 'ċereali komuni ġew irrappurtati minn Abeysekera et al.[30].Skond is-sejbiet tagħhom, TPCs ta' estratti metanoliċi ta 'varjetajiet ta' ross abjad u aħmar varjaw minn 29.34 sa 139.79mg GAEs għal kull 100g ta 'dqiq fuq bażi ta' piż xott, filwaqt li TPCs ta ' estratti etanoliċi varjaw minn 59.78 sa 113.56mg GAEs għal kull 100g ta 'dqiq fuq bażi ta' piż xott. TPCs ta 'estratti metanoliċi ta' xgħir, qamħ, qamħ, u ħafur kienu 93.61,75.10,71.22, u 17.24mg GAEs għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott. TPCs ta 'estratti etanoliċi ta' xgħir, qamħirrum, qamħ, u ħafur kienu 68.69,52.10, 78.05, u 15.72mg GAEs għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott. Għalhekk, meta mqabbla mas-sejbiet imsemmija hawn fuq, il-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha għandhom kontenut fenoliku għoli minn ċereali komunement ikkunsmati bħal ross, qamħ, qamħ, xgħir u ħafur. Il-komposti fenoliċi huma magħrufa li huma anti-ossidanti qawwija, u għalhekk, kwalunkwe tessut tal-pjanti li jkollu dawn il-komposti jista 'jintuża potenzjalment bħala anti-ossidanti naturali effettivi [2]. Konsegwentement, il-kontenuti fenoliċi għoljin indikaw il-potenzjal antiossidant tal-varjetajiet Ravi, Rawana u Oshadha.

3.1.2.DPPH u ABTS Attivitajiet ta' Tkessiħ Radikali tal-Kation. Differenzi sinifikanti (P<0.05) were="" observed="" in="" both="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts="" (table="" 2).="" methanolic="" extracts="" of="" the="" three="" varieties="" showed="" significantly=""><0.05)dpph and="" abts="" cation="" radical="" scavenging="" activities="" when="" compared="" to="" ethanolic="" extracts.="" ravi="" and="" oshadha="" varieties="" showed="" significantly="" high=""><0.05)dpph and="" abts="" cation="" radical="" scavenging="" activities="" when="" compared="" to="" rawana.="" all="" extracts="" showed="" dose-dependent="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities.="" in="" dpph="" radical="" scavenging="" assay,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" ics="" value="" indicating="" the="" highest="" activity,="" and="" in="" abts="" cation="" radical="" scavenging="" assay,="" the="" methanolic="" extract="" of="" ravi="" had="" the="" lowest="" ics="" value="" indicating="" the="" highest="">

Pożittiv sinifikanti (P<0.05)correlations were="" observed="" between="" ic="" values="" of="" dpph="" and="" abts="" cation="" radical="" scavenging="" activities="" of="" the="" extracts(r="0.750)indicating" that="" the="" finger="" millet="" extracts="" had="" comparable="" activities="" in="" the="" two="" assays.="" the="" compounds="" present="" in="" finger="" millet="" extracts="" that="" can="" scavenge="" dpph="" radicals="" may="" be="" capable="" of="" scavenging="" abts="" cation="" radicals="" as="" well.="" almeida="" et="" al.[31]="" have="" observed="" a="" similar="" correlation="" when="" studying="" the="" antioxidant="" activities="" of="" brazilian="" fruit="" extracts.="" suriano="" et="" al.="" [32]="" also="" have="" reported="" a="" similar="" correlation="" in="" barley="" varieties,="" and="" they="" have="" attributed="" it="" to="" the="" same="" reaction="" mechanism="" on="" which="" both="" assays="" rely.="" a="" good="" antioxidant="" should="" be="" capable="" of="" quenching="" free="" radicals="" [33,34].="" therefore,="" the="" ability="" to="" scavenge="" dpph="" and="" abts="" cation="" radicals="" indicated="" the="" antioxidant="" potential="" of="" ravi,="" rawana,="" and="" oshadha="" finger="" millet="">

3.1.3.Kapaċità ta 'Assorbenza Radikali ta' l-Ossiġenu. Kien hemm differenzi sinifikanti (P<0.05) in="" scavenging="" oxygen="" radicals="" among="" the="" ravi,="" rawana,="" and="" oshadha="" varieties(table="" 3), and="" the="" orac="" value="" of="" oshadha="" was="" significantly="" higher=""><0.05)than that="" of="" the="" ravi="" and="" rawana="" varieties.="" in="" raw-ana,="" the="" ethanolic="" extract="" showed="" a="" significantly="" high=""><0.05) orac="" value="" when="" compared="" to="" the="" methanolic="" extract.="" there="" were="" no="" significant="" differences="" (p≥0.05)between="" the="" orac="" values="" of="" ethanolic="" and="" methanolic="" extracts="" in="" the="" ravi="" and="" oshadha="">

Il-valuri ORAC taċ-ċereali komuni kienu rrappurtati fi studji preċedenti [30]. Il-valuri ORAC ta' estratti metanoliċi ta' varjetajiet ta' ross abjad u aħmar varjaw minn 150.61 sa 439.64mg ekwivalenti għal Trolox għal kull 100g ta' dqiq fuq bażi ta' piż xott, filwaqt li l-valuri ORAC ta' estratti etanoliċi varjaw minn 108.32 sa 272.08mg ekwivalenti għal kull Trolox. 100g ta 'dqiq fuq bażi ta' piż xott.Valuri ORAC ta 'estratti metanoliċi ta' xgħir, qamħ, qamħ, u ħafur kienu 357.51, 322.00,249.91, u 50.71mg Trolox ekwivalenti għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott. Il-valuri ORAC ta 'estratti etanoliċi ta' xgħir, qamħ, qamħ, u ħafur kienu 244.33,263.90,84.04, u 73.20mg Trolox ekwivalenti għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott. Għalhekk, meta mqabbla mas-sejbiet imsemmija hawn fuq, il-varjetajiet ta 'millieġ tas-swaba' Ravi, Raw-ana u Oshadha jippossjedu kapaċità ogħla li jneħħu r-radikali tal-perossil milli ċ-ċereali l-oħra kkunsmati b'mod komuni bħal ross, qamħ, u qamħ, xgħir u ħafur. Peress li l-kapaċità ta 'estratt li jneħħi radikali fiżjoloġiċi bħal radicals peroxyl hija indikatur importanti tal-kapaċità antiossidant tiegħu [33,34], dawn is-sejbiet indikaw il-potenzjal antiossidant tal-varjetajiet Ravi, Rawana u Oshadha.

3.1.4.Attività Chelating tal-Ion tal-ħadid. Kien hemm differenzi sinifikanti (P<0.05) in="" fic="" activities="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts="" (table="" 3).="" methanolic="" extracts="" showed="" significantly="" high=""><0.05)activities when="" compared="" to="" ethanolic="" extracts="" of="" all="" finger="" millet="" varieties.="" therefore,="" methanol="" extraction="" may="" be="" more="" efficient="" compared="" to="" ethanol="" extraction="" when="" extracting="" com-pounds,="" with="" ficability,="" from="" finger="" millet="" varieties.="" ferrous="" ions="" can="" initiate="" and="" spread="" many="" radical="" generating="" reactions,="" and="" consequently,="" chelating="" ferrous="" ions="" are="" important="" to="" prevent="" redox-active="" metal="" catalysis-associated="" ros="" generating="" reactions[35].="" therefore,="" the="" ability="" to="" chelate="" ferrous="" ions="" reflected="" the="" antioxidant="" potential="" of="" the="" ravi,="" rawana,="" and="" oshadha="">

3.1.5.Qawwa Antiossidanti Ferrika li Tnaqqas. Kien hemm differenzi sinifikanti (P<0.05) in="" frap="" values="" of="" ravi,="" rawana,="" and="" oshadha="" varieties="" and="" between="" the="" extracts(table="" 3).="" methanolic="" extracts="" showed="" significantly="" high=""><0.05)frap values="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" three="" varieties,="" oshadha="" showed="" a="" significantly="" high=""><0.05)frap value="" when="" compared="" to="" ravi="" and="" rawana.="" since="" the="" reducing="" capacity="" of="" an="" extract="" is="" an="" important="" indicator="" of="" its="" antioxidant="" capacity="" [2],="" these="" results="" provided="" evidence="" for="" the="" antioxidant="" potential="" of="" ravi,="" raw-ana,="" and="" oshadha="">

Il-valuri FRAP ta' estratti metanoliċi ta' varjetajiet ta' ross abjad u aħmar varjaw minn 61.79 sa 743.18mg Trolox ekwivalenti għal kull 100g ta' dqiq fuq bażi ta' piż xott, filwaqt li l-valuri FRAP ta' estratti etanoliċi varjaw minn 139.36 sa 255.34mg Trolox f'ekwivalenti xott għal kull dqiq. bażi tal-piż. Valuri FRAP ta 'estratti metanoliċi ta' xgħir, qamħ, qamħ, u ħafur kienu 199.45,177.95,81.06, u 61.74mg Trolox ekwivalenti għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott. Valuri FRAP ta 'estratti etanoliċi ta' xgħir, qamħ, qamħ, u ħafur kienu 107.21,84.27,49.05, u 26.60mg Trolox ekwivalenti għal kull 100g ta 'dqiq, rispettivament, fuq bażi ta' piż xott [30]. Għalhekk, meta mqabbla mas-sejbiet imsemmija hawn fuq, il-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha jippossjedu kapaċitajiet ogħla biex inaqqsu l-joni ferriku meta mqabbla mar-ross, qamħ, qamħ, xgħir u ħafur. Peress li t-tnaqqis tal-kapaċità ta 'estratt huwa indikatur importanti tal-kapaċità antiossidant tiegħu [2], il-kapaċità li jitnaqqsu l-joni tal-ferrika indikat il-potenzjal antiossidant tal-varjetajiet Ravi, Rawana u Oshadha.

3.2.Proprjetajiet Anti-infjammatorji tal-Varjetajiet tal-Millieġ tas-Saba '

3.2.1.A5-LOX Attività Inibitorja tal-Enżimi.A5-L-attivitajiet ta' inibizzjoni tal-enżimi LOX tal-estratti etanoliċi u metanoliċi tal-varjetajiet Ravi, Rawana, u Oshadha huma mogħtija fit-Tabella 4. Iż-żewġ estratti tal-varjetajiet ta' millieġ bi tliet swaba huma kapaċi jinibixxu l-azzjoni katalitika tal-A. 5-Enżima LOX b'mod li jiddependi mid-doża. Estratti metanoliċi tat-tliet varjetajiet urew b'mod sinifikanti (P<0.05) high="" a5-lox="" enzyme="" inhibitory="" activities="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" six="" finger="" millet="" extracts,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" icso="" value="" indicating="" the="" highest="" a5-lox="" enzyme="" inhibitory="">

Il-valuri tal-ICC tal-attivitajiet inibitorji tal-enzimi A5-LOX tal-estratti kienu korrelatati b'mod negattiv ma' TPCs (r=-0.788, P<0.05)and tfcs(r="-0.399,"><0.05)of the="" extracts,="" indicating="" the="" involvement="" of="" finger="" millet="" phenols,="" including="" flavonoids,="" in="" inhibiting="" the="" catalytic="" action="" of="" a5-lox="" enzyme.="" perera="" et="" al.="" [2]="" have="" observed="" a="" similar="" correlation="" in="" sri="" lankan="" medicinal="" plants,="" and="" they="" have="" reported="" that="" anti-a5-lox="" activity="" is="" proportional="" to="" the="" polyphenol="" and="" flavonoid="" contents.="" this="" is="" supported="" by="" the="" fact="" that="" most="" of="" the="" a5-lox="" enzyme="" inhibitors="" are="" phenols,="" and="" furthermore,="" the="" most="" potent="" a5-lox="" enzyme="" inhibitors,="" including="" baicalein,="" are="">

Kien hemm korrelazzjonijiet pożittivi qawwija bejn il-valuri ICs tal-attivitajiet inibitorji tal-enzimi A{{{0}}LOX u l-valuri ICs0 ta' DPPH(r{=0}.840, P<0.05)and abts="" cation="" (r="0.660,"><0.05)radical scavenging="" activities="" of="" all="" extracts.="" these="" correlations="" indicated="" the="" involvement="" of="">antiossidanti, li huma preżenti fl-estratti tal-millieġ tas-swaba', fl-inibizzjoni tal-enzima A{5-LOX billi jeliminaw l-intermedji radikali li huma ġġenerati waqt l-azzjoni katalitika tal-enzima A{5-LOX. Atomu tal-ħadid nonheme huwa preżenti fis-sit attiv tal-lipoxygenases. Ir-reazzjoni katalitika tal-lipoxygenases tinvolvi ossidazzjoni ta 'elettron wieħed fl-atomu tal-ħadid tas-sit attiv li taqleb bejn Fe2 plus u Fe3 flimkien ma' stati redox. Għalhekk, molekuli b'funzjonalitajiet li jnaqqsu l-ħadid u li jnaqqsu l-jone ferriku jistgħu jaġixxu bħala inibituri potenti ta' A5-LOX[2,37].Fl-istudju preżenti, ġew osservati wkoll korrelazzjonijiet negattivi qawwija bejn il-valuri ICs ta' A{{9} }Attivitajiet inibitorji tal-enzimi LOX u valuri FRAP (r=-0.873, P<0.05)as well="" as="" fic="" activities(r="-0.752,"><0.05)of all="" extracts="" suggesting="" that="" reducing="" the="" ferric="" ions="" and="" chelating="" the="" ferrous="" ions="" as="" potential="" mechanisms="" of="" finger="" millet="" extracts="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" a5-lox="">

3.2.2. Attività ta' Inibizzjoni ta' Enżimi XO.Kemm estratti etanoliċi kif ukoll metanoliċi tal-varjetajiet tal-millieġ tas-swaba 'Ravi, Rawana u Oshadha kienu kapaċi jinibixxu l-azzjoni katalitika tal-enzima xanthine oxidase b'mod dipendenti fuq id-doża li jindika l-anti-infjammatorjipotenzjal tat-tliet varjetajiet (Tabella 4). Estratti metanoliċi esibiti b'mod sinifikanti (P<0.05)high xo="" enzyme="" inhibitory="" activities="" when="" compared="" to="" ethanolic="" extracts.="" among="" the="" six="" finger="" millet="" extracts,="" the="" methanolic="" extract="" of="" oshadha="" had="" the="" lowest="" icso="" value="" indicating="" the="" highest="" xo="" enzyme="" inhibitory="">

Il-valuri ICC tal-attivitajiet inibitorji tal-enzimi XO tal-estratti kollha wrew b'mod sinifikanti (P<0.05) negative="" correlations="" with="" tpcs(r="-0.744)," tfcs(r="-0.489)," frap="" values="" (r="-0.793)," and="" fic="" activities(r="-0.906)indicating" the="" involvement="" of="" antioxidants,="" which="" are="" present="" in="" finger="" mil-let="" extracts,="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" enzyme.="" during="" the="" catalytic="" action="" of="" xo,="" superoxide="" anions="" and="" hydrogen="" peroxide="" are="" formed="" [38].="" the="" significant=""><0.05)positive correlations="" between="" ic="" values="" of="" xo="" enzyme="" inhibitory="" activities="" and="" ics.values="" of="" dpph="" (r="0.826)" and="" abts="" cation="" (r="0.790)radical" scavenging="" activities="" of="" all="" extracts="" suggested,="" scavenging="" radical="" intermediates="" including="" ros,="" which="" are="" generated="" during="" the="" catalytic="" action="" of="" the="" enzyme,="" as="" a="" potential="" mechanism="" of="" xo="" enzyme="">

3.2.3.Attività Inibitorja tal-Enżimi Hyaluronidase. Kemm estratti etanoliċi kif ukoll metanoliċi tal-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha kienu kapaċi jinibixxu l-azzjoni katalitika tal-enzima hyaluronidase (Figura 1). Fost it-tliet varjetajiet, Oshadha wera l-ogħla attività inibitorja tal-enzima hyaluronidase għalkemm statistikament ma kinitx sinifikanti (P ​​akbar minn jew ugwali għal 0.05) meta mqabbla maż-żewġ varjetajiet l-oħra. Ma kien hemm l-ebda differenzi sinifikanti (P ​​akbar minn jew ugwali għal 0.05) bejn l-attivitajiet inibitorji tal-enzima hyaluronidase tas-sitt estratti, u kienu b'mod sinifikanti (P<0.05)lower than="" the="" hyaluronidase="" enzyme="" inhibitory="" activity="" of="" the="" reference="" standard,="" tannic="" acid="" which="" showed="" 99.16%="" inhibition="" at="" 0.5mg/ml="" concentration.="" girish="" et="" al.="" [7]="" and="" sahasrabudhe="" and="" deodhar="" [27]="" have="" reported="" that="" polyphenols="" are="" good="" hyaluronidase="" inhibitors.="" furthermore,="" the="" reference="" standard="" used="" in="" this="" assay,="" tannic="" acid,="" is="" also="" a="" type="" of="" polyphenol="" [27].="" in="" the="" present="" study="" also,="" hyaluronidase="" enzyme="" inhibitory="" activities="" of="" the="" extracts="" showed="" a="" positive="" correlation="" with="" tpcs(r="0.387)," confirming="" the="" involvement="" of="" finger="" millet="" phenols="" in="" inhibiting="" the="" catalytic="" action="" of="" the="" hyaluronidase="">

3.2.4.Attività Inibitorja tal-Fqigħ Ossidattiv. L-abbiltajiet ta 'l-estratti etanoliċi u metanoliċi tal-varjetajiet ta' millieġ tas-swaba 'Ravi, Rawana u Oshadha biex jinibixxu l-fqigħ ossidattiv indott minn zymosan fid-demm uman sħiħ, u r-riżultati indikaw li ż-żewġ estratti tal-varjetajiet ta' millieġ bi tliet swaba huma kapaċi jinibixxi t-tifqigħ ossidattiv fid-demm sħiħ tal-bniedem b'mod dipendenti mid-doża (Tabella 4). Estratti metanoliċi esibiti b'mod sinifikanti (P<0.05) high="" inhibition="" when="" compared="" to="" ethanolic="" extracts.="" when="" compared="" with="" ibuprofen,="" methanolic="" extracts="" had="" a="" moderate="" oxidative="" burst="" inhibitory="" potential="" in="" whole="" human="" blood.="" all="" extracts="" were="" further="" evaluated="" for="" their="" oxidative="" burst="" inhibitory="" activity="" in="" isolated="" polymorphonuclear="" neutrophils(table="" 4).="" similar="" to="" the="" results="" of="" the="" study="" with="" whole="" human="" blood,="" all="" extracts="" showed="" dose-dependent="" oxidative="" burst="" inhibitory="" activities="" in="" poly-morphonuclear="" neutrophils,="" and="" methanolic="" extracts="" exhibited="" significantly=""><0.05)high inhibition="" when="" compared="" to="" ethanolic="" extracts.="" according="" to="" the="" results="" of="" the="" present="" study,="" both="" ethanolic="" and="" methanolic="" extracts="" of="" finger="" millet="" varieties="" exhibited="" a="" high="" oxidative="" burst="" inhibition="" in="" isolated="" polymorphonuclear="" neutrophils="" when="" compared="" with="" ibuprofen.="" methanolic="" extracts="" of="" ravi,="" rawana,="" and="" oshadha="" exhibited="" 8.5,5.2,="" and="" 5.6="" times="" higher="" activities,="" respectively,="" for="" oxidative="" burst="" inhibition="" in="" human polymorphonuclear="" neutrophils="" when="" compared="" to="" ibuprofen.="" this="" may="" be="" attributed="" to="" the="" significantly="" high=""><0.05) tpcs="" and="" tfcs(table="" 1)="" of="" methanolic="" extracts="" of="" the="" three-finger="" millet="">

TPCs tal-estratti nstabu li kienu korrelatati b'mod negattiv mal-valuri IC5 ta' inibizzjoni tal-fqigħ ossidattiv fid-demm uman sħiħ (r=-0.431) u newtrofili polimorfonukleari umani (r=-0.528, P<0.05). similarly,="" tfcs="" of="" the="" extracts="" were="" found="" to="" be="" negatively="" correlated="" with="" ic5so="" values="" of="" oxidative="" burst="" inhibition="" in="" whole="" human="" blood="" (r="-0.715,"><0.05)and human="" polymorphonuclear="" neutrophils(r="-0.619,"><0.05). these="" correlations="" indicated="" the="" involvement="" of="" finger="" millet="" phenols,="" including="" flavonoids,="" in="" oxidative="" burst="" inhibition.="" the="" significant=""><0.05)negative correlations="" between="" frap="" values="" of="" the="" extracts="" and="" oxidative="" burst="" inhibitory="" activity="" in="" whole="" human="" blood="" (r="-0.541)" and="" human="" polymorphonuclear="" neutrophils="" (r="-0.602)as" well="" as="" fic="" activities="" of="" the="" extracts="" and="" ics.values="" of="" oxidative="" burst="" inhibition="" in="" whole="" human="" blood(r="-0.985)and" human="" polymorphonuclear="" neutrophils(r="-0.959)suggested" that="" antioxidant="" compounds="" which="" are="" present="" in="" the="" finger="" millet="" extracts="" are="" responsible="" for="" inhibiting="" the="" oxidative="" burst.="" there="" were=""><0.05) positive="" correlations="" between="" ics="" values="" of="" abts="" cation="" radical="" scavenging="" activities="" and="" icsovalues="" of="" oxidative="" burst="" inhibitory="" activities="" in="" whole="" human="" blood="" (r="0.556)" and="" human="" polymorphonuclear="" neutrophils(r="0.652).In" addition,="" there="" were="" strong="" positive="" correlations="" between="" ic="" values="" of="" dpph="" radical="" scavenging="" activities="" and="" icsvalues="" of="" oxidative="" burst="" inhibitory="" activities="" in="" whole="" human="" blood(r="0.889,"><0.05)and human="" polymorphonuclear="" neutrophils(r="0.815,"><0.05 ).="" these="" correlations="" indicated="" scavenging="" radicals="" as="" the="" prominent="" mechanism="" in="" oxidative="" burst="" inhibition.="" antioxidants,="" which="" have="" the="" potential="" to="" inhibit="" ros-induced="" oxidative="" burst,="" can="" serve="" as="" effective="" anti-inflammatory="" agents="" [1,="" 8].="" therefore,="" these="" results="" provided="" evidence="" for="" the="" anti-inflammatory="" potential="" of="" ravi,="" rawana,="" and="" oshadha="" varieties.="" oxidative="" burst="" inhibitory="" activities="" provided="" evidence="" for="" the="" anti-inflammatory="" potential="" of="" ravi="" rawana,="" and="" oshadha="" varieties="" while="" indicating="" the="" potential="" application="" of="" methanolic="" and="" ethanolic="" extracts="" of="" ravi,="" rawana,="" and="" oshadha="" finger="" millet="" varieties="" in="" the="" prevention="" and="" management="" of="" ros-induced="" inflammatory="" conditions="" as="" natural="" sources="" of="" anti-inflammatory="" drug="">

4. Konklużjonijiet

Dan huwa l-ewwel studju li jiżvela proprjetajiet anti-infjammatorji in vitro ta' kwalunkwe estratt ta' kwalunkwe varjetà ta' millieġ tas-swaba' kkultivat fis-Sri Lanka bl-użu ta' A5-LOX, XO, hyaluronidase, u assaġġi inibitorji tal-fqigħ ossidattiv.A5-LOX u l-attivitajiet inibitorji ta 'l-enzima xanthine oxidase ta' varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha rriflettew l-applikazzjoni potenzjali fil-prevenzjoni ta 'diversi disturbi infjammatorji akkumpanjati mill-azzjonijiet katalitiċi ta' l-enzimi A5-LOX u XO. Attivitajiet inibitorji promettenti ta 'tifqigħ ossidattiv fid-demm uman sħiħ u newtrofili polimorfonukleari iżolati wrew li kemm estratti etanoliċi kif ukoll metanoliċi tal-varjetajiet ta' millieġ tas-swaba 'Ravi, Rawana, u Oshadha jistgħu jkunu sorsi sinjuri ta' komposti bijoattivi inibitorji tal-fqigħ ossidattiv u konsegwentement jirriflettu l-applikazzjoni potenzjali ta 'Ravi, Rawana. , u varjetajiet ta 'millieġ tas-swaba' Oshadha fil-prevenzjoni u l-ġestjoni ta 'kundizzjonijiet infjammatorji indotti minn ROS. Minħabba s-sejbiet dwar proprjetajiet anti-infjammatorji, il-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha jistgħu jintużaw b'mod effettiv bħala sorsi naturali ta 'kandidati għal drogi anti-infjammatorji, li jissodisfaw id-domanda għal aġenti anti-infjammatorji ġodda li huma derivati ​​minn sorsi naturali. Se jsiru aktar studji bbażati fuq iċ-ċelluli biex jivverifikaw dawn is-sejbiet.

Dan l-istudju żvela wkoll il-proprjetajiet antiossidanti tal-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha. Skont is-sejbiet, il-varjetajiet ta 'millieġ tas-swaba' Ravi, Rawana u Oshadha fihom ammonti għoljin b'mod sinifikanti ta 'komposti fenoliċi inklużi flavonoids. It-tliet varjetajiet ta 'millieġ tas-swaba' għandhom il-potenzjal li jneħħuradikali ħielsainklużi DPPH, cation ABTS, u radikali ta 'l-ossiġnu, biex jitnaqqsu joni tal-metall u joni tal-metall kelat li jtemmu reazzjonijiet li jiġġeneraw radikali. Dawn is-sejbiet indikaw li l-varjetajiet ta 'millieġ tas-swaba' Ravi, Raw-ana u Oshadha jistgħu jkunu sorsi tajbin ta 'antiossidanti. Konsegwentement, il-konsum ta’ varjetajiet ta’ millieġ tas-swaba’ Ravi, Rawana u Oshadha fuq bażi regolari jista’ jkollu rwol importanti fil-prevenzjoni u l-ġestjoni tad-dieta ta’stress ossidattiv-mard assoċjat. Meta mqabbla mal-proprjetajiet antiossidanti rrappurtati ta 'ċereali ikkunsmati b'mod komuni inklużi ross, qamħ, qamħ, xgħir, u ħafur, il-varjetajiet ta' millieġ tas-swaba 'Ravi, Rawana u Oshadha għandhom proprjetajiet antiossidanti għoljin. Dawn is-sejbiet jaġixxu bħala gwida utli fl-għażla tal-ikel għall-konsum ta 'kuljum u huma ta' għajnuna fit-teħid ta 'deċiżjonijiet għall-industrija tal-ikel biex tieħu l-vantaġġ tad-dqiq tal-millieġ tas-swaba' bħala alternattiva jew suppliment għal dqiq taċ-ċereali ieħor.

B'mod kollettiv, is-sejbiet tal-istudju preżenti espandew l-għarfien attwali tal-proprjetajiet bijoattivi tal-varjetajiet ta 'millieġ tas-swaba' li bħalissa huma kkultivati ​​u kkunsmati fis-Sri Lanka, u enfasizzaw il-proprjetajiet terapewtiċi tagħhom. Se jsiru aktar studji biex jiġu identifikati l-komposti fenoliċi speċifiċi li huma responsabbli għal kull attività.