Effetti Ta' Partijiet Estratti Differenti Ta' Cistanche Deserticola Fuq Effetti Ipogliċemiċi Ta' Ġrieden Iswed Db / Db Ⅲ

3 Riżultati sperimentali

3.1 Riżultati tad-determinazzjoni tal-kontenut ta' partijiet estratti differenti ta' Cistanche deserticola/Cistanche deserticola

Imkejla skond il-kondizzjonijiet taħt "2.3", ir-riżultati tad-determinazzjoni tal-kontenut ta 'partijiet estratti differenti ta' Cistanche deserticola uTartschula deserticolahuma murija fit-Tabella 1.

ESTRAT TA' CISTANCHE ORGANIKU NATURALI B'30% ECHINACOSIDE U 12% ACTEOSIDE

3.2 Riżultati tal-kejl tal-indiċi farmakoloġiku

3.2.1 Osservazzjoni ġenerali

Il-perjodu tal-esperiment tal-annimali kien ta '4 ġimgħat, u l-piż tal-ġisem tas-sawm tal-ġrieden suwed kien imkejjel kull ġimgħa. Ir-riżultati huma murija fit-Tabella 2. Mill-bidu sat-tmiem tal-esperiment tal-gavage, il-piż tal-ġrieden suwed fil-grupp tal-mudell kompla jiżdied, li kien konsistenti mal-karatteristiċi taż-żieda fil-piż fil-mudell tad-dijabete tat-tip 2. Il-piż tal-firien fil-grupp ta' kontroll normali baqgħu bażikament l-istess. Wara ġimgħa 1 ta 'amministrazzjoni intragastrika ta' ġrieden suwed db/db fi gruppi ta 'amministrazzjoni differenti, meta mqabbla mal-grupp mudell, iż-żieda fil-piż tal-firien f'kull grupp ta' amministrazzjoni tjiebet fi gradi differenti, fosthom l-effett ta 'titjib tal-glycosides totali ta' tartrum kien il- l-akbar. B'mod sinifikanti (P<0.05); after 2 weeks and 4 weeks of intragastric administration of db/db black mice in different administration groups, the body weight of Amaranth oligosaccharide improved significantly (P<0.05 or P<0.01) . The above results show that different extracted parts of Cistanche deserticola and Branco deserticola have the effect of reducing the body weight of db / db black mice. The results are shown in Table 2.

3.2.2 Effetti ta' partijiet estratti differenti ta' Cistanche deserticola fuq il-livelli ta' glucose fid-demm ta' ġrieden suwed db/db

Iz-zokkor fid-demm tal-ġrieden suwed db/db fil-grupp ta' kontroll normali nżamm f'livell normali (4.3 ~ 5.7 mmol·L-1), filwaqt li z-zokkor fid-demm tal-ġrieden suwed fil-grupp mudell kompla jiżdied (P<0.01). Compared with the model group, after 1 week of administration, the total polysaccharides, total oligosaccharides, and total glycosides of Cistanche deserticola group and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Tartistan camphora group were reduced (P<0.01); after 2 weeks of administration, After 3 weeks of administration, the total glycosides FBG of tartschistanches were reduced (P＜0.05); after 3 weeks of administration, the total polysaccharides and total oligosaccharides FBG of tartans were reduced (P＜0.05 or P＜0.01); After taking the medicine for 4 weeks, the total polysaccharides, total oligosaccharides of Cistanche deserticola, and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Cistanche deserticola were reduced (P <0.05). The results are shown in Table 3.

Fit-test tat-tolleranza tal-glukożju orali (ara Tabella 4), il-livell medju tal-glukożju fid-demm tal-grupp ta 'kontroll normali ta' ġrieden suwed laħaq quċċata fi 30 minuta, u mbagħad niżel malajr għal livelli normali. Madankollu, il-livell medju ta 'glukożju fid-demm tal-ġrieden suwed fil-grupp mudell kompla jibqa' f'livell għoli wara li laħaq il-quċċata fi 30 minuta. Meta mqabbel mal-grupp mudell, il-glukożju fid-demm tal-ġrieden suwed f'kull grupp ta 'amministrazzjoni wera xejra 'l isfel fi gradi differenti wara li laħaq l-ogħla valur, fosthom il-polysaccharide totali, l-oligosaccharide totali u l-grupp ta' glycosides totali tal-grupp Tartschalna campestris naqas aktar malajr. Barra minn hekk, skont l-AUC ikkalkulat ta’ kull grupp, ir-riżultati wrew li l-AUC tal-ġrieden suwed f’kull grupp ta’ amministrazzjoni kienet aktar baxxa minn dik tal-grupp mudell, u l-polysaccharides totali ta’ Cistanche deserticola u l-glycosides totali ta’ Tartistan deserticola naqqsu l- AUC tal-ġrieden suwed f'2 punti ta' ħin (90 u 120 min) wara l-għoti tal-glukożju.Valur tal-glukożju fid-demm(P<0.05 or P<0.01), total oligosaccharides of Cistanche deserticola and total polysaccharides of Tartschalisnaqqas il-glukożju fid-demmvalur wara l-għoti tal-glukożju għal 120 minuta (P<0.05), total oligosaccharides of Tartschalis. Itnaqqsu t-tliet punti ta’ ħin(0, 90, u 120 min) wara l-amministrazzjoni tal-glukożju u l-erja taħt il-kurva (P<0.05 or P<0.01). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the OGTT ability of db / db black mice.

Meta mqabbel mal-grupp ta 'kontroll normali, il-indiċi tar-reżistenza għall-insulina(HOMA-IR) uLivelli ta' HbA1c tal-ġrieden suwedfil-grupp mudell żdied (P<0.05), and the pancreatic islet β cell secretion index (HOMA-β) level decreased. Compared with the model group, the serum HbA1c content of the Cistanche deserticola oligosaccharide group, Cistanche deserticola polysaccharide group, Tartschistanche deserticola oligosaccharide group and Tartschistanche deserticola total glycosides group was significantly reduced (P <0.05); , the HOMA-IR index of the alcoholic acid agaricus oligosaccharide group and the alcoholic acid agaricus total glycosides group was significantly reduced (P <0.05); after 4 weeks of intervention in the administration group, compared with the model group, the black mice in each administration group The secretion index levels of pancreatic β-cells increased to varying degrees. The results are shown in Table 5.

L-insulina hija ormon tal-proteina li hija prinċipalment sintetizzata u mnixxija minn ċelloli tal-gżejjer fit-tessut tal-frixa. Jipparteċipa fil-metaboliżmu tal-glukożju tal-ġisem u jżomm il-bilanċ taz-zokkor fid-demm tal-ġisem. Il-livelli ta' INS fis-serum jistgħu jirriflettu l-effett tat-trattament tad-dijabete. Jista' jidher mit-Tabella 5 li meta mqabbel mal-grupp normali, il-livell INS tal-ġrieden suwed fil-grupp mudell kien imnaqqas b'mod sinifikanti (P<0.01). After 4 weeks of intervention in the administration group, compared with the model group, the fasting serum insulin levels of black mice in each administration group increased to varying degrees, among which the total glycosides of Cistanche deserticola and Tartscholaris had the most obvious increase. The results showed that each administration group could increase the serum insulin level of db/db black mice and promote the secretion of insulin by pancreatic β-cells.

3.2.3 Effetti ta' partijiet estratti differenti ta' Cistanche deserticola/Cistanche deserticola fuq il-livell ta' stress ossidattiv tal-fwied ta' ġrieden suwed db/db

Il-livell MDA fil-fwied tal-ġrieden suwed fil-grupp mudell kien ogħla b'mod sinifikanti minn dak fil-grupp normali (P<0.05). After 4 weeks of intervention in the medication group, compared with the model group, the MDA levels of each medication group decreased to varying degrees (P < 0.05), among which Cistanche deserticola total polysaccharides, Cistanche deserticola total oligosaccharides, Cistanche deserticola total glycosides, and wine The level of MDA in the liver tissue of black mice treated with total glycosides of Cistanche deserticola and total oligosaccharides of Cistanche deserticola showed the most obvious decrease (P<0.05 or P<0.01). The results showed that Cistanche deserticola had a certain effect on reducing the MDA level in the liver tissue of db/db black mice, and the improvement effect was better than that of the alcoholic acid deserticola administration group.

L-attività SOD fit-tessut tal-fwied tal-ġrieden suwed fil-grupp mudell kienet aktar baxxa minn dik fil-grupp ta 'kontroll normali (P<0.05). When different extract parts of Cistanche deserticola and Tartscholaris are administered orally, the SOD activity in the liver tissue of db / db black mice can be increased. Compared with the model group, each administration group can restore SOD activity, among which the total glycosides of Cistanche deserticola group are different. There is statistical significance (P<0.05). The results are shown in Table 6.

3.2.4 Effetti ta' partijiet estratti differenti ta' Cistanche deserticola/Cistanche deserticola fuq il-metaboliżmu tax-xaħam tal-fwied ta' ġrieden suwed db/db

Sabiex jiġi evalwat l-effett ta 'Cistanche deserticola/Tartrol deserticola fuq il-livell tal-metaboliżmu tal-lipidi fis-serum ta' ġrieden suwed db/db, ġew skoperti markaturi relatati mal-metaboliżmu tal-lipidi tad-dijabete fl-esperiment. Jista’ jidher mit-Tabella 7 li meta mqabbel mal-grupp ta’ kontroll normali, il-livell ta’ LDL-C fis-serum tal-grupp mudell żdied b’mod sinifikanti (P<0.05), while the HDL-C level was decreased. After 4 weeks of drug intervention, compared with the model group, the serum TG, TC and LDL-C levels of the Cistanche deserticola total polysaccharide, total oligosaccharide and total glycosides group and the Tartistan deserticola total polysaccharide, total oligosaccharide and total glycosides group were significantly lower than those of the model group. The levels of HDL-C decreased to varying degrees and the levels of HDL-C increased, among which the Cistanche deserticola total glycosides group had the effect of improving TC levels (P<0.05). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the lipid metabolism disorder of db / db black mice to a certain extent.

3.2.5 Effetti ta' partijiet estratti differenti ta' Cistanche deserticola/Cistanche deserticola fuq UA, Cr u mALB fil-plażma u l-awrina ta' ġrieden suwed db/db

Wara 4 ġimgħat ta 'amministrazzjoni, meta mqabbla mal-grupp mudell, il-livelli UA, Cr u mALB tal-polisakkaridi totali ta' Cistanche deserticola, oligosakkaridi totali u l-grupp ta 'glycosides alkoħoliċi totali ta' Cistanche deserticola tnaqqsu fi gradi differenti. Minbarra t-tnaqqis ta’ Cr, UA u mALB fl-awrina (P＜0.05 jew P＜0.01), il-grupp tal-polisakkarid tal-agaricus tal-aċidu alkoħoliku tejjeb il-livelli ta’ UA, Cr u mALB fl-awrina (P<0.01). 05 jew P<0.01), ethanol can improve the UA level in plasma (P<0.05), and the total glycosides of Cistanche cantnaqqas l-UA fil-plażma, Cr u mALB livelli ta 'ġrieden iswed db / db sa ċertu punt.

3.2.6 Effetti ta' prodotti pproċessati differenti u partijiet estratti ta' Cistanche deserticola fuq l-istopatoloġija tal-frixa u l-kliewi ta' ġrieden suwed db/db

Ma kien hemm l-ebda ħsara patoloġika lit-tessut tal-frixa tal-ġrieden suwed fil-grupp ta 'kontroll normali. Truf ċari tal-gżejjer kienu viżibbli. Iċ-ċelloli tal-gżejjer kienu ovali u rranġati b'mod ordnat fiċ-ċitoplasma. In-nuklei kienu tondi u l-gżejjer kienu aktar numerużi u akbar fid-daqs. It-tessut tal-frixa tal-ġrieden suwed fil-grupp mudell ma kellux gżejjer kompluti. It-truf tal-gżejjer ma kinux ċari u l-forma ma kinitx kompluta. Iċ-ċelloli tal-gżejjer kienu rranġati b'mod diżordinat, b'numru żgħir, atrofija, u ħsara severa lill-frixa. Meta mqabbel mal-firien fil-grupp mudell, wara 4 ġimgħat ta 'amministrazzjoni intragastrika ta' partijiet ta 'estrazzjoni differenti ta' Cistanche deserticola/Tartarchus deserticola, it-tessut tal-gżejjer tal-frixa tal-grupp ta 'amministrazzjoni f'kull sit ta' estrazzjoni rkupra parzjalment, il-volum taċ-ċelluli tal-gżejjer żdied fi gradi differenti, u ċ-ċelloli tal-gżejjer kellhom konfini ċari. , l-arranġament diżordinat huwa mtejjeb. Fosthom, il-grupp ta 'glycosides totali ta' Cistanche deserticola u Tartschalne deserticola wera l-aħjar irkupru.

grupp ta' kontroll normali

Cistanche deserticola polysaccharide grupp

Tartschalna campestris polysaccharide grupp

grupp mudell

Cistanche deserticola oligosaccharide grupp

Grupp tartracean oligosaccharide

grupp ta' kontroll pożittiv

Grupp ta 'glycosides totali Cistanche deserticola

Grupp ta 'glycosides totali ta' Tarthola cistanche

Figura 3 Effetti ta 'partijiet estratti differenti ta' Cistanche deserticola/Cistanche deserticola fuq patoloġija pankreatika ta 'ġrieden suwed db/db (H&E, 200 ×)

Ma kien hemm l-ebda ħsara ovvja lit-tessut tal-kliewi tal-ġrieden suwed fil-grupp ta 'kontroll normali. L-istruttura glomerulari, il-forma u l-proporzjon mal-kavità taċ-ċisti glomerulari kienu normali. Il-ħitan tat-tubuli prossimali u distali kienu rqaq u ħoxnin, u d-daqs tal-lumen kien normali. L-istruttura, il-forma, u d-daqs tal-glomeruli tal-ġrieden suwed fil-grupp mudell kienu irregolari, u l-glomeruli atrofija u l-kliewi

Il-kavità tal-bużżieqa tisparixxi, it-tubuli prossimali u distali għandhom ħxuna tal-ħajt irregolari, daqsijiet differenti tal-lumen, konfini mhux ċari, nefħa u nekrożi, u ammont kbir ta 'infiltrazzjoni taċ-ċelluli infjammatorji. Meta mqabbel mal-firien fil-grupp mudell, wara 4 ġimgħat ta 'amministrazzjoni intragastrika ta' partijiet estratti differenti ta 'Cistanche deserticola/Tamarococcus deserticola, it-tessut renali tal-gruppi ta' amministrazzjoni f'kull parti estratta tjieb, u l-istruttura, il-forma u d-daqs glomerulari kienu qrib għan-normal, u t-tubuli prossimali Il-ħajt tat-tubuli distali huwa rqiq, id-daqs tal-lumen jitjieb, in-nefħa titnaqqas, u ammont żgħir ta 'infiltrazzjoni taċ-ċelluli infjammatorji xorta jista' jidher f'xi partijiet. Fosthom, il-grupp tal-polisakkarid Cistanche deserticola u l-grupp tal-polisakkarid deserticola alkoħoliku rkupraw l-aħjar.

3.2.7 L-espressjoni tal-kollaġen tat-tip IV fit-tessut tal-kliewi ta 'ġrieden suwed db / db minn partijiet estratti differenti ta' Cistanche deserticola / Litose deserticola hija simili għal dik tal-grupp normali.

Figura 4. Effetti ta 'partijiet estratti differenti ta' Cistanche deserticola/Tamarococcus deserticola fuq il-patoloġija renali ta 'ġrieden suwed db/db (H&E, 200 ×). L-espressjoni tal-proteina tal-kollaġen tat-tip IV fil-kliewi tal-ġrieden suwed fil-grupp mudell inbidlet b'mod sinifikanti (P＜ 0.05). Meta mqabbel mal-grupp mudell, iż-żona medja tal-matriċi tal-membrana renali tal-ġrieden suwed f'kull grupp ta 'amministrazzjoni żdiedet. Fosthom, l-espressjoni tal-kollaġen tat-tip IV fil-grupp polisakkarid Cistanche deserticola, il-grupp oligosaccharide tartschistanche deserticola, u l-grupp ta 'glikosidi totali tartschistanche deserticola tjiebet, filwaqt li l-kliewi Ma kien hemm l-ebda differenza statistikament sinifikanti fl-espressjoni tal-kollaġen tat-tip IV fil-membrana tal-kantina.

grupp ta' kontroll normali

Cistanche deserticola polysaccharide grupp

Cistanche deserticola polysaccharide grupp

grupp mudell

Cistanche deserticola oligosaccharide grupp

Cistanche deserticola oligosaccharide grupp

grupp ta' kontroll pożittiv

Grupp ta 'glycosides totali Cistanche deserticola

Grupp ta 'glycosides totali Cistanche deserticola

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